微流控芯片分选富集循环肿瘤细胞的研究进展

近年来，循环肿瘤细胞(CTCs)研究得到了越来越多的关注，许多研究报告已经证实其在肿瘤转移的早期诊断、治疗方案选择、个体化治疗及探索肿瘤转移机制等方面具有潜在的价值，然而CTCs在循环系统中的含量极低，这成为限制其临床相关应用的主要难点。微流控芯片技术具有低成本、快速、高通量及操作简单等优势，利用微流控芯片可实现CTCs的高速、高回收率、高纯度的分选富集，近年来得到广泛的关注。本文综述了近年来在微流控芯片内进行CTCs分选富集的研究并探讨了各种方法的优缺点，并在本研究团队的研究基础上进行了展望。

Advances in isolation and enrichment of circulating tumor cells in microfluidic chips

The research of circulating tumor cells (CTCs) has drawn increasing attention in recent years, because its potential value in the early diagnosis of cancers, management of clinical treatment, development of personalized medicine, and exploring the mechanism of metastasis. However, the difficulty in using CTCs lies in their extremely low concentrations. Recently, microfluidic devices have shown the potential to efficiently isolate and detect rare CTCs in cancer patients. A variety of on-chip blood analysis has been demonstrated by several groups. The advantages of microfluidics include low cost, short reaction times, high throughput, and ease of use. Recently, a variety of microfluidic devices have been developed to CTCs isolation and enrichment which can realize high capture efficiency, high purity and high throughput. In this article, we review some of the recent works in microfluidic CTCs isolation and enrichment devices and discuss in what field should be done next based on our researches.