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Studies on alkaline protease produced by Bacillus sp. NG312
Authors:S Jasvir  Navdeep Gill  Gina Devasahayam  Debendra K Sahoo
Institution:(1) Institute of Microbial Technology, Sector 39-A, 160 036 Chandigarh, India;(2) Present address: Department of Chemical Engineering, Massachusetts Institute of Technology, Room No. 56-454, 02139 Cambridge, MA
Abstract:An alkalophilic hyperproducer of alkaline protease, Bacillus sp. NG312, was isolated, and the enzyme showed maximum activity at pH 11.0 and 60°C. The temperature optimum was increased by 10°C in presence of Ca2+. The crudeenzyme was found to have half-life of 11 d at 37°C and maximum stability at pH 9.0–10.0. It also exhibited very good stability in presence of detergent components and some locally available commericial detergent powders.
Keywords:Alkaline protease            Bacillus            characterization  detergent compatibility
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