Studies on alkaline protease produced by Bacillus sp. NG312 |
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Authors: | S Jasvir Navdeep Gill Gina Devasahayam Debendra K Sahoo |
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Institution: | (1) Institute of Microbial Technology, Sector 39-A, 160 036 Chandigarh, India;(2) Present address: Department of Chemical Engineering, Massachusetts Institute of Technology, Room No. 56-454, 02139 Cambridge, MA |
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Abstract: | An alkalophilic hyperproducer of alkaline protease, Bacillus sp. NG312, was isolated, and the enzyme showed maximum activity at pH 11.0 and 60°C. The temperature optimum was increased
by 10°C in presence of Ca2+. The crudeenzyme was found to have half-life of 11 d at 37°C and maximum stability at pH 9.0–10.0. It also exhibited very
good stability in presence of detergent components and some locally available commericial detergent powders. |
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Keywords: | Alkaline protease Bacillus characterization detergent compatibility |
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