Grafting of silica with sulfobetaine polymers via aqueous reversible addition fragmentation chain transfer polymerization and its use as a stationary phase in HILIC |
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Authors: | Erika Wikberg Jeroen J. Verhage Camilla Viklund Knut Irgum |
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Affiliation: | 1. Department of Chemistry, Umeå University, Umeå, Sweden. Fax: +46-90-7867655;2. Merck SeQuant AB, Umeå, Sweden |
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Abstract: | Porous silica particles of 3 μm diameter and 100 Å nominal pore size were first activated for vinylic polymerization by functionalization with 3-methacryloyloxypropyl trimethoxysilane (MAPTMS) and thereafter dressed with zwitterionic grafts of the sulfoalkylbetaine type in the “grafting through” fashion by polymerizing 3-(2-(N-methacryloyloxyethyl)-N,N-dimethylammonio)propane sulfonate (SPE), using either free radical polymerization or controlled reversible addition fragmentation chain transfer polymerization (RAFT). Particles polymerized using RAFT had a lower overall coating which seemed to be more evenly distributed in the pore volume. Both approaches resulted in columns with similar separation properties in HILIC mode. |
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Keywords: | Graft polymerization HILIC HPLC Porous silica RAFT Stationary phase |
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