Use of immobilized triazine dyes in the purification of DNA topoisomerase I (Topo I) and terminal deoxynucleotidyl transferase (TdT) from calf thymus. |
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| Authors: | A Markauskas V Tiknius R Marcisauskas |
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| Affiliation: | Institute of Applied Enzymology FERMENTAS, Lithuania, U.S.S.R. |
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| Abstract: | The interaction between TdT and Topo I, and twelve various triazine dyes immobilized on Sepharose CL-6B was studied. Yellow lightproof 2KT-Sepharose and Bordeaux 4ST-Sepharose were used to purify TdT and Topo I, respectively. The principal role of copper ions, complexed to the dye molecules, in the dye-protein interaction was evaluated. |
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