Liquid chromatography-mass spectrometry of hydroxy and non-hydroxy fatty acids as amide derivatives. |
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Authors: | M Ikeda T Kusaka |
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Affiliation: | Department of Biochemistry, Kawasaki Medical School, Okayama, Japan. |
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Abstract: | A useful method for analyzing fatty acids by liquid chromatography-mass spectrometry with an atmospheric-pressure chemical-ionization interface system has been developed. The sensitivity of six kinds of palmitamide derivatives monitored by a single ion of [M+H]+ was, in decreasing order: N-n-propylamide greater than anilide greater than N,N-diethylamide, amide greater than N,N-diphenylamide greater than N-1-naphthylamide. Individual fatty acids were identified from a mixture of amide derivatives of authentic fatty acids from C16:0 to C30:0 on a mass chromatogram. This method was used to detect both hydroxy and non-hydroxy fatty acids. Many kinds of fatty acid, including hydroxy fatty acids of the rat brain, were detected in a single run. |
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