解偶联剂CCCP对莱茵衣藻光照产氢过程的调控

本文研究莱茵衣藻在含硫和缺硫连续光照条件下, 不同浓度的CCCP对PSⅡ光化学效率、 光照产氢、 光合放氧以及光能的吸收和转换效率等的影响和调控规律.

Regulation of Hydrogen Production by Uncoupler CCCP in Green Algae Chlamydomonas reinhardtii

 Uncoupler,  carbonylcyanide-m-chlorophenylhydrazone[CCCP],  can markedly inhibit the photochemical activity of photosystem Ⅱ and repress the rate of photosynthetic oxygen evolution.  As a result,  the anaerobic condition can promptly induce hydrogenase expression and accelerate hydrogen photoproduction under the  continuous illumination by Chlamydomonas reinhardtii.  When C. reinhardtii cultured in TAP medium and treated with 0,  5 and   15 μmol/L CCCP under the continuous illumination,  the photochemical activity of algae could not be obviously inhibited.  But 15 and 20 μmol/L CCCP could markedly depress the photochemical activity and substantial amount of hydrogen gas was photoproduced.  The photochemical activity of C. reinhardtii cultured with TAP-S at all concentrations of CCCP under the continuous llumination was distinctly inhibited and the cultures photoproduced hydrogen gas rapidly.  The algae of C. reinhardtii cultured with TAP and TAP-S medium,  different concentrations of CCCP altered the process of hydrogen metabolism and the efficiency of utilization and conversion of solar energy by the center of PSⅡ.