Molecularly selective field-effect transistors for determining nicotinamide adenine dinucleotide and its phosphates

Molecularly selective field-effect transistors are developed for determining oxidized and reduced forms of nicotinamide adenine dinucleotides (NAD⁺ and NADH) and their phosphates (NAD(P)⁺ and NAD(P)H). Copolymers of acrylamide and acrylamidophenylboric acid imprinted with corresponding nucleotides are used as sensing elements of sensors. After washing out a nucleotide, a cavity corresponding to the surface of the imprinted nucleotide is retained and ensures the selectivity of the sensor. At the same time, sensors containing any of the nucleotides of interest in solutions demonstrate almost equal sensitivities of 10^?7 M. The properties of the sensors are explained by the structure of the nucleotides and the composition of the membranes.