| 毛细管气相色谱测定胶原蛋白和卵清蛋白氨基酸 |
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| 引用本文： | 师治贤,张金霞,陈耀祖,薛敦渊,李兆琳. 毛细管气相色谱测定胶原蛋白和卵清蛋白氨基酸[J]. 分析测试学报, 1990, 0(1) |
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| 作者姓名： | 师治贤 张金霞 陈耀祖 薛敦渊 李兆琳 |
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| 作者单位： | 中国科学院西北高原生物研究所，中国科学院西北高原生物研究所，兰州大学分析测试中心，兰州大学分析测试中心，兰州大学分析测试中心 西宁，西宁 |
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| 摘 要： | 讨论了纳克量级(ppb)的18种氨基酸在石英毛细管住气相色谱的分离和定量测定。用此法对胶原蛋白和卵清蛋白水解氨基酸进行了定量。结果的精确度为0.8～7.3%。氨基酸的衍生化采用N-TFA-butyl系统,分二步完成,第一步在酸性介质中用亚丁醇酯化;第二步是用三氟醋酐酰化。氨基酸回收率在85%以上。蛋白质水解在6N恒沸点盐酸中充氮气,在145±2℃加热4小时完成。
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| 关 键 词： | 胶原蛋白 卵清蛋白 氨基酸 石英毛细管气相色谱 |
Capillary Gas Chromatographic Cetermination of Amino-acids from Collagen and Egg White Protein |
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| Shi Zhixian,Zhang Jinxia,Chen Yaozu,Xue Dunyuan,Li Zeaolin Northwest Plateau Institute of Biology Academia Instrument Analysis and Research Center of Lanzbou University. Capillary Gas Chromatographic Cetermination of Amino-acids from Collagen and Egg White Protein[J]. Journal of Instrumental Analysis, 1990, 0(1) |
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| Authors: | Shi Zhixian Zhang Jinxia Chen Yaozu Xue Dunyuan Li Zeaolin Northwest Plateau Institute of Biology Academia Instrument Analysis Research Center of Lanzbou University |
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| Affiliation: | Shi Zhixian;Zhang Jinxia;Chen Yaozu;Xue Dunyuan;Li Zeaolin Northwest Plateau Institute of Biology Academia Instrument Analysis and Research Center of Lanzbou University |
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| Abstract: | Amino-acids from acid hydrolysis of collagen and egg white protein were esterfied in ac-idic solution with n-butyl alcohol and then treated with trfluoroacetic anhydryde. The 18 am-ino-acids in the acylation solution thus obtatained were determined by capillary gas chromato-graphy. |
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| Keywords: | Collagen eggwhite protein amino acids capillary GC_f |
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