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| SP700大孔树脂纯化酸枣仁中三萜总皂苷的研究 | |
| 引用本文： | 丁轲,崔莹,陆晶晶,袁其朋.SP700大孔树脂纯化酸枣仁中三萜总皂苷的研究[J].离子交换与吸附,2011,27(1). |
| 作者姓名： | 丁轲 崔莹 陆晶晶 袁其朋 |
| 作者单位： | 1. 北京化工大学生命科学与技术学院,北京生物加工过程重点实验室,北京,100029;北京农学院食品科学系,北京,102206 2. 北京农学院食品科学系,北京,102206 3. 北京化工大学生命科学与技术学院,北京生物加工过程重点实验室,北京,100029 |
| 基金项目： | 北京市优秀人才培养资助项目(No.20081D0502100229); 农产品加工及贮藏工程北京市重点建设学科(No.PXM2009_014207_078172) |
| 摘 要： | 从7种极性不同的大孔树脂中筛选出一种对酸枣仁三萜总皂苷具有良好吸附和解吸性能的树脂—三菱SP700树脂。静态实验中,该树脂的最高吸附容量可达131.0mg皂苷/g树脂,皂苷在树脂表面的吸附符合Langmuir模型,用体积分数为90%的乙醇解吸得率最高可达97.8%;动态实验中,样液在膨胀床模式下上样,树脂的平均动态吸附容量为15mg皂苷/g树脂,上样后,用体积分数分别为50%和90%的乙醇溶液进行分段洗脱,洗脱得率最高可达到95.1%,经过纯化的皂苷提取物的含量提高了4倍。SP700大孔吸附树脂作为一种对酸枣仁中三萜总皂苷进行纯化的介质具有良好的性能和应用价值。 |
| 关 键 词： | 酸枣仁 大孔树脂 吸附 洗脱 皂苷 |
STUDY ON PURIFICATION OF TOTAL TRITERPENOID SAPONINS IN Zizyphi spinosi semen BY USING SP700 MACROPOROUS RESIN |
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| DING Ke,CUI Ying,LU Jingjing,YUAN Qipeng.STUDY ON PURIFICATION OF TOTAL TRITERPENOID SAPONINS IN Zizyphi spinosi semen BY USING SP700 MACROPOROUS RESIN[J].Ion Exchange and Adsorption,2011,27(1). | |
| Authors: | DING Ke CUI Ying LU Jingjing YUAN Qipeng |
| Institution: | DING Ke1,2 CUI Ying2 LU Jingjing1 YUAN Qipeng1 1.Key Laboratory of Bioprocess of Beijing,Beijing University of Chemical Technology,Beijing 100029,China 2.Department of Food Science,Beijing University of Agriculture,Beijing 102206,China |
| Abstract: | In this study,Mitsubishi SP700 resin with high performance of adsorption and desorption was selected from 7 different types of macroporous adsorption resins.In static state,the highest adsorption capacity was up to 131.0mg saponins/g resin;Langmuir isothermal adsorption equation was suitable for describing the adsorption process of saponins on SP700;90%(v/v) EtOH solution was used as desorption reagent and desorption efficiency was over 95%.In dynamic state,expanded bed was adopted for uploading,the average... |
| Keywords: | Zizyphi spinosi semen Macroporous resin Adsorption Elution Saponins |
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