发育中花生胚离体萌发和蛋白酶活性变化及脱落酸的影响

发育过程中花生胚氨肽醇有两个活性高峰，与贮藏蛋白质的两个合成高峰相吻合，发育晚期有微弱的羧肽酶活性出现，脱落酸能抑制离体胚萌发，抑制内肽酶和羧肽酶活性，但不抑制氨肽酶活性。发育早期离体胚萌发时贮藏蛋白质降解依赖于羧肽酶、Ａｃｔ－Ｄ和ＣＨＭ处理结果表明羧肽酸在成熟胚已预存但活性处于抑制状态。

Germination and Changes of Activitiesof Peptidases During Development of PeanutEmbryos in Vitro and Effect of Abscisic Acid on Them

Aminopeptidase activity had two peaks during development of peanut(Arachis hypogaea L. ) embryos, which coincided with the synthesis of storage pro-teins. A weak activity peak of carboxypeptidase emerged in the late stage of develop-ment of embryo. Aminopeptidase might have an important role in modifying the struc-ture of peptides of storage proteins. Abscisic acid inhibited germination of embryos invitro, decreased activities of endopeptidase and carboxypeptidase, but aminopeptidaseactivitiy was not affected. Degradation of storage proteins in germinating embryos invitro during development was dependent upon carboxypeptidase. Endogenous ABA con-tent declined in germinating embryos. Results from Act-D and CHM treatment showedthat carboxypeptidase existed in cells of matured embryos but its activity was mainly in-hibited.