Choice of procedure conditions for radioimmunoassay of aflatoxin B1 using125I as a marker and dextrancoated charcoal as a separation matrix |
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| Authors: | L. Fukal J. Prosek P. Rauch Z. Sova J. Káš |
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| Affiliation: | (1) Department of Biological Sciences, University of Agriculture, 160 21 Prague 6, Czechoslovakia;(2) Laboratory of Cooperative Farm, Pot!["ecaron"]("/content/b36233q51652j637/xlarge283.gif") hy, 285 63 Tupadly, Czechoslovakia;(3) Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia |
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| Abstract: | Assay conditions for the radioimmunoassay for aflatoxin B1 using125I-radiolabel and dextran-coated charcoal for the separation of free and bound radioligand were optimized. Casein was chosen as the best protecting protein /in contrast with human serum albumin,  -globulin and gelatine/. The most suitable incubation conditions are at 4°C for 18 h in darkness, radioligand sorption on the dextrancoated charcoal takes place 30 min at 4°C and the antiserum is diluted in order to reach zero specific binding in the range between 35 and 50%. |
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