Use of a terbium chelate as label in spectrofluorimetric determinations of protein traces |
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| Authors: | Jerzy Siepak |
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| Affiliation: | Department of Instrumental Analysis, Faculty of Chemistry, A. Mickiewicz University, 60-780 Poznań Poland |
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| Abstract: | A spectrofluorimetric method for the determination of protein traces using the terbium complex formed with diethylenetriaminepentamethanephosphonic acid and p-aminosalicyclic acid as reagents is described. The method has been applied to the trace determination of simple proteins at concentrations down to 10?11 M in the pH range 2.5–11. |
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