Measurement of nitrate gradients with an ion-selective microelectrode |
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Authors: | Dirk de Beer Jean-Pierre RA Sweerts |
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Institution: | Department of Chemical Engineering and Biotechnological Centre, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam The Netherlands;Limnological Institute, Rijksstraatweg 6, 3631 AC Nieuwersluis The Netherlands |
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Abstract: | Nitrate-selective microelectrodes (1-μm tip diameter) with a liquid membrane ion exchanger were prepared. The response of the electrode is linear from 10?1 to 10?5 M nitrate and the response time is 30 s. Selectivity coefficients are of the same order as specified for macroelectrodes. Measurements in sediments of a mesotrophic lake and measurements in spherical agar gels with immobilized denitrifying bacteria are presented. |
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