Enzyme electrodes for the sugar substitute aspartame |
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Authors: | George G Guilbault GJ Lubrano J-M Kauffmann GJ Patriarche |
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Institution: | Universal Sensors, P.O. Box 736, New Orleans, LA 70148 U.S.A.;Pharmaceutical Institute, Free University of Brussels, Boulevard du Triomphe, 1050 Brussels Belgium |
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Abstract: | A sensor of aspartame (l-aspartyl-l-phenylalanine methyl ester) is prepared by chemical immobilization of l-aspartase on an ammonia-selective electrode. Semi logarithmic response (E vs. log C) was observed in the 1 × 10?3?1 × 10?2 M range with a slope of ?30 mV/decade. The sensor is stable for more than eight days. The probe is successfully used for the assay of aspartame in commercially available sweeteners. |
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