Amperometric probe for 3-hydroxybutyrate with immobilized 3-hydroxybutyrate dehydrogenase |
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Authors: | G Palleschi HS Rathore M Mascini |
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Institution: | Dipartimento di Scienze e Tecnologie Chimiche II, Università degli Studi di Roma, Via O. Raimondo, 00173 Roma Italy;Istituto di Chimica Analitica Università degli Studi di Firenze, Via G. Capponi 9, 50121 Firenze Italy |
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Abstract: | The enzyme 3-hydroxybutyrate dehydrogenase is immobilized on a graphite electrode suitable for determination of NADH. Of the several procedures tested for the immobilization, direct adsorption on the electrode surface was most satisfactory, with useful lifetimes of up to 3 days. The best calibration graphs for the NADH, and for 3-hydroxybutyrate were obtained at pH 7.5 (phosphate buffer). 3-Hydroxybutyrate was determined in the range 5–100 μmol l?1 at pH 7.5 with good precision. Interferences are discussed. |
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