Integrated biosensor for glucose and galactose |
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| Authors: | K Yokoyama K Sode E Tamiya I Karube |
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| Institution: | Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsutacho, Midori-ku, Yokohama 227 Japan;Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153 Japan |
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| Abstract: | A glucose microsensor based on quinoprotein glucose dehydrogenase (GDH) and electron mediators is described. It is unaffected by the concentration of dissolved oxygen. Its calibration graph is linear below 10 mg dl ?1. The insensitivity to oxygen arises because the concentration of the oxidized forms of the mediators is insufficient to oxidize the reduced form of GDH. An integrated microsensor for glucose and galactose based on GHD and galactose oxidase was constructed. Glucose and galactose concentrations were determined from the current increase due to oxidation of the mediators or the current decrease due to reduction of oxygen. |
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