Fluorimetric determination of calcium in serum with calcein : Complexation of Calcein with Calcium and Alkali Metals |
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| Authors: | Mavroudis A Demertzis |
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| Institution: | Inorganic and Analytical Chemistry Laboratory, Department of Chemistry, University of Ioannina, Dourouti, Ioannina Greece |
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| Abstract: | Calcium (0–50 μM) is determined by measuring the fluorescence of its calcein complexes in strongly alkaline solutions. The effects of alkali metal complexation are described, and the nature of the calcium complexes is investigated. The method is applied to serum. |
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