Alcohol-FET sensor based on a complex cell membrane enzyme system |
| |
| Authors: | Eiichi Tamiya Isao Karube Yasushi Kitagawa Minoru Ameyama Koji Nakashima |
| |
| Affiliation: | Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 227 Japan;Central Research Laboratories, Asahi Breweries Ltd., 2-13-1 Ohmori-kita, Ohta-ku, Tokyo 143 Japan;Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753 Japan;Department of Laboratory Medicine, St. Luke''s International Hospital, 10-1 Akashi-cho, Chuoku, Tokyo 104 Japan |
| |
| Abstract: | An alcohol -FET sensor was developed by use of a complex enzyme system in a cell membrane and an ion-sensitive field effect transistor (ISFET). The cell membrane of Gluconobacter suboxydans IFO 12528, which converts ethanol to acetic acid, was immobilized on the gate of an ISFET with calcium alginate gel coated with nitrocellulose. This ISFET (1), a reference ISFET without the cell membrane (ISFET 2) and an Ag/AgCl reference electrode were placed in 5 mM Trismalate buffer (pH 5.5, 25°C), and the differential output between ISFETS 1 and 2 was measured. The output of the sensor was stabilized by adding pyrroloquinoline quinone. The response time was ca. 10 min., and there was a linear relationship between the differential output voltage and the ethanol concentration up to 20 mg l?1. The output of the sensor was stable for 40 h below 30°C. The sensor responded to ethanol, propan- 1-ol and butan- 1-ol, but not to methanol, propan-2-ol and butan-2-ol. The sensor was used to determine blood ethanol. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
