Bioluminescence flow sensor for determination of creatine kinase activity in blood |
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Authors: | S Girotti ML Cascione S Ghini G Carrea R Bovara A Roda R Motta R Petilino |
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Institution: | Istituto Scienze Chimiche, Università di Bologna, Via S. Donato 15, 40127 Bologna Italy;Istituto Chimica Ormoni, CNR, Via M. Bianco 9, 20131 Milan Italy;Istituto di Chimica Analitica, Università di Messina, Salita Sperone, 98100 S. Agata di Messina Italy;Laboratorio Centralizzato, Ospedale S. Orsola, Via Massarenti 9, 40128 Bologna Italy |
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Abstract: | A bioluminescent flow sensor was developed for the assay of creatine kinase (CK) using firefly luciferase immobilized on a nylon coil. The CK-catalysed reaction of creatine phosphate with ADP took place in a cuvette before the injection into the bioluminescent detector coil. The response was linear from 0.1 to 100 U l? at 25°C. An advantage of the flow sensor is a detection limit of less than 0.1 U l?1, which, together with a high precision, allows determination of the CK activity in blood sera in about 5 min. The intra- and inter-assay reproducibilities (RSD) were less than 10% and the recovery range was 86–110%. The results agreed well with those obtained with a spectrophotometric method and with the normal reference values. |
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