1,2-bis(4-methoxyphenyl)ethylenediamine as a fluorogenic reagent for reducing carbohydrates |
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Authors: | Yoshihiko Umegae Hitoshi Nohta Yosuke Ohkura |
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Institution: | Faculty of Pharmaceutical Sciences, Kyushu University 62, Maidashi, Higasi-ku, Fukuoka 812 Japan |
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Abstract: | Eight 1,2-diarylethylenediamines in the meso- or DL-form produce fluorescence when heated with reducing carbohydrates in an alkaline medium. Of the diamines, meso-1,2-bis(4-methoxyphenyl)ethylenediamine is the most favourable reagent for reducing carbohydrates, including 2- deoxy sugars, amino sugars and sialic acids. The reagent permits the fluorimetric determination of reducing carbohydrates at concentrations as low as 0.2–0.9 nmol ml?1. |
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