1,2-Diarylethylenediamines as fluorogenic reagents for catecholamines |
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Authors: | Yoshihiko Umegae Hitoshi Nohta Yosuke Ohkura |
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Affiliation: | Faculty of Pharmaceutical Sciences, Kyushu University 62, Maidashi, Higashi-ku, Fukuoka 812 Japan |
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Abstract: | Twenty-eight 1,2-diarylethylenediamines were investigated in a search for highly sensitive fluorogenic reagents for catecholamines (norepinephrine, epinephrine and dopamine). They all react with epinephrine used as a model catecholamine under mild conditions (pH 6.5–6.8, 37–50°C) in the presence of potassium hexacyanoferrate (III) to produce fluorescence. Of the compounds tested, 1,2-bis(3,4-dimethoxyphenyl)ethylenediamine, 1,2-bis(4-methoxyphenyl)ethylenediamine and 1,2-bis (4-ethoxyphenyl)ethylenediamine, all in the meso-form, are the most sensitive for all the catecholamines. 1,2-Diphenylethylenediamine, 1,2-bis(4-methoxyphenyl)ethylenediamine and 1,2-bis (4-methylphenyl) ethylenediamine in the dl-form are more sensitive to dopamine than the corresponding compounds in the meso-form. Catecholamines can be determined at concentrations as low as 10–15 pmol ml?1 by using those compounds as reagents. The reactions of other catechol compounds are reported. Other types of biologically important compounds do not interfere. |
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