Chromatographic and luminescence properties of a 7-aminocoumarin derivative with peroxyoxalate chemiexcitation

A new derivatizing reagent for amines, luminarine 1, is described, and tested as its pentylamine derivative. Chromatographic separation in both normal- and reversed-phase systems was achieved. With fluorescence detection, the limit of detection was in the subpicomole range. With the optimization procedure for chemiluminescence detection, the limit of detection was 1 fmol in the normal-phase system (using bis (2,4-dinitrophenyl) oxalate and hydrogen peroxide), and 6 fmol in the reversed-phase system (using bis (2,4,6-trichlorophenyl) oxalate and hydrogen peroxide ), but the coefficient of variation was lower for the latter system (3.4% vs. 5.8%).