Amperometric bienzyme electrode for l-carnitine |
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| Authors: | Maurice Comtat Muriel Galy Philippe Goulas Jerome Souppe |
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| Institution: | Laboratoire de Chimie Physique et Electrochimie, UA CNRS no. 192, Université Paul Sabatier, 118 Route de Narbonne, 31062 Toulouse Cédex France;Groupe de Recherche de Lacq, Société Nationale Elf Aquitaine, BP 34, 64170 Artix France |
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| Abstract: | An electrochemical sensor with two soluble enzymes allows assay of 0.1–10 mM, l- carnitine with an accuracy of 2%. The assay takes about 2 min. The first enzyme, carnitine dehydrogenase, catalyzes the oxidation of l-carnitine by NAD+. The NADH formed is oxidized by hexacyanoferrate(III) in a reaction catalyzed by diaphorase, and the hexacyanoferrate(II) produced is oxidized by electrolysis at 0.3 V. The sensor can be used for several days. |
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