Alcohol sensor based on membrane-bound alcohol dehydrogenase |
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Authors: | Yasushi Kitagawa Katsuaki Kitabatake Izumi Kubo Eiichi Tamiya Isao Karube |
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Institution: | Central Research Laboratories, Asahi Breweries Ltd., 2-13-1 Ohmori-kita, Ohta-ku, Tokyo 143 Japan;Fuji Electric Corporate Research and Development Ltd., 2-2-1 Nagasaka, Yokosuka 240-01 Japan;Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153 Japan |
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Abstract: | Ethanol is determined by a sensor system using purified, immobilized mernbrane-bound alcohol dehydrogenase frorn Gluconobacter suboxydans, attached to a platinum disk electrode (3 mm diameter), and covered with a dialysis membrane. Hexacyanoferrate (III) is used as the redox acceptor. To correct for the influence of interfering substances, this alcohol sensor is compensated by a control electrode which has no immobilized enzyme. The potential of these platinum electrodes was set at + 350 mV vs. Ag/AgCl. Linearity was observed in the range 0.1–5 mM ethanol, the response time was less than 5 min, the maximum sensitivity was obtained at 45°C and the optimum pH was in the range 4.5–5.5. The sensitivity decreased to 80% of the initial value after 1 month at 30°C. When the alcohol sensor system was applied to the determination of ethanol in alcoholic beverages, a good correlation was obtained between the results and those obtained by gas chromatography. |
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