Glucose-sensitive field-effect transistor with a membrane containing co-immobilized gluconolactonase and glucose oxidase |
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Authors: | Yoshio Hanazato Ken-Ichi Inatomi Mamiko Nakako Satoru Shiono Mitsuo Maeda |
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Institution: | Central Research Laboratory, Mitsubishi Electric Corporation 1-1, Tsukaguchi-Honmachi 8-Chome, Amagasaki, Hyogo 661 Japan |
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Abstract: | A glucose-sensitive field-effect transistor (FET) with a two-enzyme membrane containing gluconolactonase and glucose oxidase is investigated. The two-enzyme membrane (ca. 1 μm thick) is formed on the ion-sensitive gate of the FET by photopolymerization. The gluconolactonase used was a partially purified product prepared from crude glucose oxidase by gel filtration. A glucose sensor with only purified glucose oxidase has little response for glucose, but the co-immobilization of gluconolactonase and glucose oxidase considerably enhanced the response amplitude of the glucose sensor. The composition of the two-enzyme/photopolymer solution is optimized; gluconolactonase with an activity at least twice that of glucose oxidase is necessary. The linear calibration graph extends from 0.2 to 2 mM glucose. |
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