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Di-isopropyl fluorophosphate electrode based on squid nerve tissue
Authors:Shunichi Uchiyama  Yukihiro Sato  Yoshinobu Tofuku  Shuichi Suzuki
Affiliation:Department of Environmental Engineering, Saitama Institute of Technology, 1690 Fusaiji, Okabe, Saitama 369-02 Japan
Abstract:Di-isopropyl fluorophosphatase is contained in the giant axon of squid nerve tissue. This tissue can be coupled with a fluoride ion-selective electrode to produce an electrode selective to di-isopropyl fluorophosphate. The semilogarithmic calibration curve at pH 7.1 is linear over the range 2 × 10?6?7 × 10?3 M (RSD < 3% ) with a slope of about 44 mV/decade. The detection limit is 8 × 10?6 M. The electrode lifetime (with constant response characteristics) is about 16 days.
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