Capillary zone electrophoresis for the analysis of peptides synthesized by recombinant DNA technology |
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Authors: | H Lüdi E Gassmann H Grossenbacher W Märki |
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Institution: | Central Analytical Department, Ciba-Geigy Ltd., Basel Switzerland;Biotechnology Department, Ciba-Geigy Ltd., Basel Switzerland |
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Abstract: | Capillary zone electrophoresis is applied to investigate the recombinant insulin-like growth factor and recombinant hirudin. During the production of these peptides in S. cerevisiae, byproducts with small variations in the structure of the polypeptide chain are obtained. The different peptides are separated in a fused silica capillary and detected on-column by ultraviolet absorption or fluorescence. Separation times are 10–40 min. The excellent separation efficiencies obtained indicate that capillary zone electrophoresis is complementary to liquid chromatography in the analysis of these peptides. |
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