A catechol electrode based on spinach leaves |
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| Authors: | Shunichi Uchiyama Minoru Tamata Yoshinobu Tofuku Shuichi Suzuki |
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| Institution: | Department of Environmental Engineering, Saitama Institute of Technology, 1690, Fusaiji, Okabe, Saitama 369-02 Japan |
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| Abstract: | Minced spinach leaf (Spinacea oleracea) has a high activity of catechol oxidase (dimerizing) (EC 1.1.3.14), which is utilized for the determination of catechol by coupling the spinach tissue with a Clark oxygen electrode. The calibration graph for catechol is linear over the range 2×10?5–8×10?4 M (RSD 3%). The sensor retains its enzyme activity for at least 18 days. 4-Methylcatechol and glycolate interfere; glucose and ascorbate do not. |
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