Quantification of several monohydroxylated metabolites of polycyclic aromatic hydrocarbons in urine by high-performance liquid chromatography with fluorescence detection |
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Authors: | Yu?Wang Email author" target="_blank">Wenbing?ZhangEmail author Yulian?Dong Ruifang?Fan Guoying?Sheng Jiamo?Fu |
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Institution: | (1) Guangdong Key Laboratory of Environment Protection & Resources Utilization, State Key Laboratory of Organic Geochemistry, Guangzhuo Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, Guangdong, 510640, P.R. China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, P.R. China |
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Abstract: | A high-performance liquid chromatographic method with fluorescence detection has been developed to determine the urinary polycyclic
aromatic hydrocarbon metabolites 2-hydroxynaphthalene, 2-hydroxyfluorene, 9-hydroxyphenanthrene, 1-hydroxypyrene and 3-hydroxybenza]pyrene.
Solid phase extraction (SPE) was used to clean up the samples, and washing with 30% methanol was found to be the best way
to remove interferences in the matrix. The method detection limits ranged from 0.044 μg/L for 1-hydroxypyrene to 1.615 μg/L
for 3-hydroxybenza]pyrene, and the recoveries ranged between 40% for 3-hydroxybenza]pyrene and 99% for 2-hydroxynaphthalene.
The within-day relative standard deviation was lowest for 2-hydroxynaphthalene at 0.67% and went up to 2.42% for 3-hydroxybenza]pyrene,
and the between-day relative standard deviation ranged from 3.84% for 9-hydroxyphenanthrene to 10.42% for 2-hydroxyfluorene.
The correlation coefficients were between 0.9962 and 0.9998. The developed method was successfully used to analyze samples
from student volunteers in a high school. |
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Keywords: | Polycyclic aromatic hydrocarbons Metabolite Urine High-performance liquid chromatography Solid phase extraction |
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