Strategies for Enhancing Laccase Yield from <Emphasis Type="Italic">Streptomyces psammoticus</Emphasis> and Its Role in Mediator-based Decolorization of Azo Dyes |
| |
Authors: | K N Niladevi P S Sheejadevi P Prema |
| |
Institution: | (1) Biotechnology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Trivandrum, 695019, India |
| |
Abstract: | Enhanced production of laccases from Streptomyces psammoticus in solid-state fermentation was carried out using two different strategies: laccase inducers and scale-up process. Laccase
yield was enhanced by a wide range of aromatic inducers. The best inducer was pyrogallol, which yielded 116 U/g as compared
to the control (55.4 U/g). Scale-up studies in packed bed bioreactor was performed at different aeration rates. Aeration at
1.5 vvm was identified as the optimum condition for laccase production (75.4 U/g) in the column bioreactor. The enzyme yield
was enhanced further by combining the best conditions from the first two experiments. Fermentation was carried out in bioreactors
in the presence of 1 mM pyrogallol, which resulted in 3.9-fold increase in laccase yield (215.6 U/g). The role of laccase
in azo dye decolorization was evaluated in the presence of four different laccase mediators, at different concentrations.
1-Hydroxybenzotriazole (HOBT) proved to be the best mediator for S. psammoticus laccase and decolorized the azo dyes efficiently. Acid orange, Methyl orange, and Bismarck brown were decolorized at the
rates of 86%, 71%, and 75% respectively, by HOBT. |
| |
Keywords: | Laccase Aromatic inducers Packed bed reactor Laccase-mediators Dye decolorization Streptomyces psammoticus |
本文献已被 PubMed SpringerLink 等数据库收录! |
|