| Abstract: | Sharp polymer fractions have been obtained in preparative-scale gel-permeation chromatography by use of a column set packed with polystyrene gel particles of about 10 μ diameter. When 0.13 g of standard polystyrene NBS 706 was fractionated per injection, fractions with polydispersities of about 1.02–1.03 were obtained over the molecular weight range 104–106. Effects of a injection volume, solution concentration, and fraction volume on fractionation efficiency were also investigated. Polydispersities of fractions increased appreciably with increasing amounts of polymer injected. |
