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| 毛细管电泳对脑外伤脑脊髓液中兴奋性氨基酸的测定 | |
| 引用本文： | 陆豪杰,郭寅龙,梁冰,欧庆瑜.毛细管电泳对脑外伤脑脊髓液中兴奋性氨基酸的测定[J].化学学报,2002,60(9):1677-1681. |
| 作者姓名： | 陆豪杰 郭寅龙 梁冰 欧庆瑜 |
| 作者单位： | 1. 中国科学院上海有机化学研究所,上海,200032;中国科学院兰州化学物理研究所,兰州,730000 2. 中国科学院上海有机化学研究所,上海,200032 3. 中国科学院兰州化学物理研究所,兰州,730000 |
| 基金项目： | 中国博士后科学基金资助项目 |
| 摘 要： | 用毛细管电泳分离测定了脑外伤脑脊髓液中的活性氨基酸，考察了进样方式、 缓冲液浓度、有机添加剂、氨基酸的衍生条件对脑脊髓液中微量活性氨基酸测定的 影响，最终在采用外加苯甲酸作为内标的优化电泳条件下，对活性氨基酸的检测限 低于1.7 * 10~(-7)mol/L，测定结果的相对标准偏差小于2%。 |
| 关 键 词： | 电泳 氨基酸 脑脊液 |
| 修稿时间： | 2001年11月9日 |
Determination of Excitatory Amino Acids in Cerebrospinal Fluid Following Cerebral Injury by Capillary Electrophoresis |
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| LU,Hao-Jie a,b GUO,Yin-Long,a LIANG,Bing b OU,Qing-Yu,b.Determination of Excitatory Amino Acids in Cerebrospinal Fluid Following Cerebral Injury by Capillary Electrophoresis[J].Acta Chimica Sinica,2002,60(9):1677-1681. | |
| Authors: | LU Hao-Jie a b GUO Yin-Long a LIANG Bing b OU Qing-Yu b |
| Institution: | Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences.Shanghai(200032);Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences,Lanzhou(730000) |
| Abstract: | Capillary electrophoresis was developed to determine excitatory amino acids in cerebrospinal fluid following cerebral injury. The effect of injection module, concentration of buffer, organic additive, and derivative conditions on determination minute quantity of excitatory amino acids in cerebrospinal fluid was investigated. The limitation of detection was less than 1.7 * 10~(-7)mol/L under the optimum conditions with benzene carboxylic acid as an internal standard. The reproducibility was also satisfactory with the relative standard deviation less than 2%. |
| Keywords: | capillary electrophoresis excitatory amino acids cerebrospinal fluid |
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