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Microfluidic Device for the Manipulation of Microparticles for Flow Cytometry Application
Authors:Matthew Attard  David Coral  Èric Pedrol  Magdalena Aguiló  Francesc Díaz  Xavier Mateos
Institution:1. Universitat Rovira i Virgili, URV, Física i Cristal·lografia de Materials, FiCMA Marcel·lí Domingo 1, Tarragona, 43007 Spain;2. SRCiT – Service for Scientific and Technical Resources Campus Sescelades, N2 building, Universitat Rovira i Virgili, Països Catalans 26, Tarragona, Av. 43007 Spain
Abstract:This work produces a unique microfluidic device that acts as a “lab on a chip,” conducting separation, mixing, and concentration of microparticles similar to that required by cell sorting flow cytometry applications. The passive two-dimensional device shows to be successful at separating polystyrene (PS) beads between 5 and 20 µm in diameter, mixing them with an external media, and concentrating them by 250% continuously with minimal sample preparation, while still being inexpensive, and effective. By implementing the microfluidic device, the processing steps are done within seconds due to its high throughput of 2 mL min−1, wherein different hydrodynamic phenomena such as Dean's forces, inertial lift forces, and enhanced diffusion are taken advantage of.
Keywords:cell sorting  flow cytometry  labs-on-a-chip  microfluidic devices  microparticle manipulation
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