A new way to rapidly create functional, fluorescent fusion proteins: random insertion of GFP with an in vitro transposition reaction |
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| Authors: | Douglas L Sheridan Catherine H Berlot Antoine Robert Fiona M Inglis Klara B Jakobsdottir James R Howe Thomas E Hughes |
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| Affiliation: | (1) Interdepartmental Neuroscience Program, Yale University Medical School, 330 Cedar St, New Haven, CT 06520, USA;(2) Department of Cellular and Molecular Physiology, Yale University Medical School, 330 Cedar St, New Haven, CT 06520, USA;(3) Department of Pharmacology, Yale University Medical School, 330 Cedar St, New Haven, CT 06520, USA;(4) Department of Ophthalmology & Visual Science, Yale University Medical School, 330 Cedar St, New Haven, CT 06520, USA |
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| Abstract: | Background The jellyfish green fluorescent protein (GFP) can be inserted into the middle of another protein to produce a functional, fluorescent fusion protein. Finding permissive sites for insertion, however, can be difficult. Here we describe a transposon-based approach for rapidly creating libraries of GFP fusion proteins. |
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