| X射线致HeLa细胞损伤过程中NADPH氧化酶的作用 |
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| 引用本文： | 杜斌斌,刘箐,张维胜,王晓妍,贾鹏飞,王小虎,李莎,杨熊飞,张红,郭天康.X射线致HeLa细胞损伤过程中NADPH氧化酶的作用[J].原子核物理评论,2008,25(4):391-396. |
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| 作者姓名： | 杜斌斌 刘箐 张维胜 王晓妍 贾鹏飞 王小虎 李莎 杨熊飞 张红 郭天康 |
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| 作者单位： | 1 兰州大学临床医学院, 甘肃 兰州 730000; 2 中国科学院近代物理研究所, 甘肃 兰州 730000; 3 甘肃省人民医院, 甘肃 兰州 730000; 4 兰州大学生命科学学院, 甘肃 兰州 730000; 5 甘肃省肿瘤医院, 甘肃 兰州 730050; 6 兰州军区总医院, 甘肃 兰州 730050 |
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| 摘 要： | 以HeLa细胞为实验材料， 探讨了NADPH氧化酶在X射线诱导细胞损伤过程中的作用。 结果显示， 12 Gy X射线辐照后细胞内活性氧(ROS)明显增加， 在用NADPH氧化酶抑制剂处理后再辐照， 则细胞内ROS降低到未辐照水平； 同时辐照后NADPH 氧化酶细胞质亚基p47phox 在细胞质积聚并和细胞膜亚基 gp91phox 结合； Western blotting检测结果显示， NADPH 氧化酶的关键亚基 gp91phox 的表达量明显增加。 以上结果说明， NADPH氧化酶可以被X射线激活， 由其介导产生的ROS在X射线诱导HeLa细胞损伤过程中扮演重要角色。 To investigate the role of NADPH oxidase in HeLa cell lesion induced by X ray irradiation, the change of cell survival was detected with MTT assay， reactive oxygen species (ROS) was measured by fluorospectrophotometer. Immunostaining and confocal laser scanning microscopy was employed to detect the co localization of two subunit of NADPH oxidase, p47phox and gp91phox in the cell. Western blotting was used to detect the expression of gp91phox before and after X ray irradiation. After X ray irradiation， intra cellular level of ROS increased obviously. But the increase could be blocked by diphenyleneiodonium (DPI)， an inhibitor of NADPH oxidase. Meanwhile， cytosolic subunit p47phox moved to membrane and co localizated with gp91phox after irradiation. Moreover, the results also show that gp91phox increased sharply after 12 Gy X ray irradiation. Therefore， NADPH oxidase mediated production of ROS plays an important role in HeLa cell lesion induced by X ray.
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| 关 键 词： | NADPH氧化酶 活性氧 X射线 HeLa细胞 |
| 收稿时间： | 1900-01-01 |
Role of NADPH Oxidase in HeLa Cell Lesion Induced by X-ray Irradiation |
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| DU Bin-bin,LIU Qing,ZHANG Wei-sheng,WANG Xiao-yan,JIA Pen-fei,WANG Xiao-hu,LI Sha,YANG Xiong-fei,ZHANG Hong,Guo Tian-kang.Role of NADPH Oxidase in HeLa Cell Lesion Induced by X-ray Irradiation[J].Nuclear Physics Review,2008,25(4):391-396. |
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| Authors: | DU Bin-bin LIU Qing ZHANG Wei-sheng WANG Xiao-yan JIA Pen-fei WANG Xiao-hu LI Sha YANG Xiong-fei ZHANG Hong Guo Tian-kang |
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| Institution: | 1 Clinical Medical College, Lanzhou University, Lanzhou 730000, China;2 Institute Of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;3 People’s Hospital of Gansu Province, Lanzhou 730000, China;4 School of Life Science, Lanzhou University, Lanzhou 730000, China;5 Gansu Tumor Hospital, Lanzhou 730050, China;6 General Hospital of Lanzhou Military Area, Lanzhou 730050, China |
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| Abstract: | To investigate the role of NADPH oxidase in HeLa cell lesion induced by X-ray irradiation,the change of cell survival was detected with MTT assay,reactive oxygen species(ROS)was measured by fluorospectrophotometer.Immunostaining and confocal laser-scanning microscopy was employed to detect the co-localization of two subunit of NADPH oxidase,p47phox and gp91phox in the cell.Western blotting was used to detect the expression of gp91phox before and after X-ray irradiation.After X-ray irradiation,intra-cellular level of ROS increased obviously.But the increase could be blocked by diphenyleneiodonium(DPI),an inhibitor of NADPH oxidase.Meanwhile,cytosolic subunit p47phox moved to membrane and co-localizated with gp91phox after irradiation.Moreover,the results also show that gp91phox increased sharply after 12 Gy X-ray irradiation.Therefore,NADPH oxidase-mediated production of ROS plays an important role in HeLa cell lesion induced by X-ray. |
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| Keywords: | NADPH oxidase reactive oxygen species X-ray HeLa cell |
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