Utilizing DART mass spectrometry to pinpoint halogenated metabolites from a marine invertebrate-derived fungus |
| |
Authors: | Watts Katharine R Loveridge Steven T Tenney Karen Media Joseph Valeriote Frederick A Crews Phillip |
| |
Affiliation: | Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, California 95064, USA. |
| |
Abstract: | Prenylated indole alkaloids are a diverse group of fungal secondary metabolites and represent an important biosynthetic class. In this study we have identified new halogenated prenyl-indole alkaloids from an invertebrate-derived Malbranchea graminicola strain. Using direct analysis in real time (DART) mass spectrometry, these compounds were initially detected from hyphae of the fungus grown on agar plates, without the need for any organic extraction. Subsequently, the metabolites were isolated from liquid culture in artificial seawater. The structures of two novel chlorinated metabolites, named (-)-spiromalbramide and (+)-isomalbrancheamide B, provide additional insights into the assembly of the malbrancheamide compound family. Remarkably, two new brominated analogues, (+)-malbrancheamide C and (+)-isomalbrancheamide C, were produced by enriching the growth medium with bromine salts. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|