Purification and Application of a Lipase from <Emphasis Type="Italic">Penicillium expansum</Emphasis> PED-03 |
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Authors: | Tang Lianghua Xia Liming Su Min Guo Huaying |
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Institution: | (1) Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou, 310027, China;(2) College of Life Sciences, Fujian Normal University, Fuzhou, 350108, China |
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Abstract: | An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with
19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was about 28 kDa, determined
by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzymatic resolution of racemic ibuprofen was carried
out by the lipase from P. expansum PED-03, and the conversion reached 46% with excellent enantioselectivity(E > 200 ), which showed a good application potential in the production of optically pure ibuprofen. |
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Keywords: | Lipase Penicillium expansum Purification Resolution Ibuprofen |
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