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Influence of ammonium chloride on growth and fatty acid production by spirulina platensis |
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| Authors: | Manabe Eichi Hirano Morio Takano Hiroyuki Ishikawa-Doi Naoko Sode Koji Matsunaga Tadashi |
| Institution: | 1.Advanced Technology Research Laboratory, Onoda Cement Co., Ltd., 2- 4- 2, Osaku, Sakura- City, 285, Chiba, Japan ;2.Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan ; |
| Abstract: | Changes in growth and fatty acid content ofSpirulina platensis were examined after transferring cells into media containing various concentrations of ammonium chloride. Photosynthetic O2 evolution rate decreased with increasing ammonium chloride concentration. Therefore, the algal growth was interrupted by ammonium chloride addition. On the other hand, total fatty acid content markedly increased after addition of ammonium chloride to a concentration of 15–50 mM and was maximized 40–48 h after addition of 25 mM ammonium chloride. The increases in palmitic and oleic acid content were especially remarkable. However, this began to decrease 48 h after the addition of 25 mM of ammonium chloride. Also, γ-linolenic acid content increased continuously during a 72-h incubation. As a result,Spirulina platensis cells containing about 2% γ-linolenic acid were obtained by ammonium chloride treatment, representing an increase of 1.5–2-fold compared to untreated cells. |
| Keywords: | Index Entries" target="_blank">Index Entries Spirulina platensis blue-green alga fatty acid γ -linolenic acid ammonium chloride |
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