Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination |
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Authors: | Nakajima Kazuki Oda Yasuo Kinoshita Mitsuhiro Masuko Takashi Kakehi Kazuaki |
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Affiliation: | Faculty of Pharmaceutical Sciences, Kinki University, Higashi-osaka, Japan. |
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Abstract: | We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A. |
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