Protein complexes/aggregates as potential cancer biomarkers revealed by a nanoparticle aggregation immunoassay |
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| Institution: | 1. Department of Mechanical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, R.O.C.;2. Advanced Institute of Manufacturing for High-Tech Innovations, National Chung Cheng University, Chia-Yi, Taiwan, R.O.C.;1. Ortopedia-Traumatologia e Chirurgia Protesica e dei reimpianti d''anca e di ginocchio, IRCCS Istituto Ortopedico Rizzoli, Via Pupilli 1, Bologna, 40136, Italy;2. Laboratorio di Tecnologia Medica, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, Bologna, 40136, Italy;3. Laboratorio di Bio Ingegneria Computazionale, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, Bologna, 40136, Italy;1. Université de Paris, INSERM U1132, 75010 Paris, France;2. Department of Medicine, Institut Mutualiste Montsouris, Paris, France;3. Department of Rheumatology, Hôpital Lariboisière, APHP Paris Nord, Paris, France;4. Université de Paris, INSERM U1132, Paris, France;1. Leiden Centre of Data Science, Leiden University, Leiden, The Netherlands;2. TiCC, Tilburg University, Tilburg, The Netherlands;3. 34, Courthope Road, Hampstead, London NW3 2LD, United Kingdom;4. eLaw@Leiden, Leiden University, Leiden, The Netherlands;1. School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China;2. Division of Laboratory in Nanfang Hospital of Nanfang Medical University, Guangzhou 510515, China |
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| Abstract: | Protein–protein interactions and protein complex/aggregate formation play an essential role in almost all biological functions and activities. Through a nanoparticle aggregation immunoassay, we discovered that some proteins are substantially more complexed/aggregated in cancer tissues than normal tissues. This study examined four biomarkers proteins, CA125, CEA (carcinoembryonic antigen), CA19-9 and PAP (prostatic acid phosphatase) in ovarian, colon and prostate tissue lysates. The most exciting results were observed from the PAP assay of prostate tissues: prostate cancer can be clearly distinguished from normal prostate and prostate with benign conditions such as BPH (benign prostate hyperplasia) based on the complex/aggregation level of PAP in prostate tissue lysates. The complex/aggregate level of a protein can be potential biomarkers for cancer detection and diagnosis. |
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