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Biocrystallization of silver and gold ions by inactive cell filtrate of Rhizopus stolonifer
Institution:1. Department of Food Science and Technology, College of Agriculture and Life Science, Chonbuk National University, Jeonju 561-756, Republic of Korea;2. Singapore-Delft Water Alliance, National University of Singapore, 2 Engineering Drive 2, Singapore 117577, Singapore;1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, China;2. Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou, China;3. State Key Laboratory of New Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China;4. Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong;1. Swedish Centre for Resource Recovery, University of Borås, 501 90 Borås, Sweden;2. Department of Molecular Biology and Genetics, Gebze Technical University, Gebze-Kocaeli 41400, Turkey;1. INBA-Instituto de Investigaciones en Biociencias Agrícolas y Ambientales / CONICET-Consejo Nacional de Investigaciones Científicas, Facultad de Agronomía, Universidad de Buenos Aires, Avda. San Marín 4453, C1417DSE Buenos Aires, Argentina;2. INQUIMAE-Instituto de Química Física de los Materiales, Medio Ambiente y Energía, y DQIAQF-Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Abstract:Microbes and their cell filtrates are known to synthesize metal nanoparticles. But maintenance of aseptic conditions and irregularly shaped and sized nanoparticles are major drawbacks of the system. In this study cell filtrate from inactive biomass of Rhizopus stolonifer was used for the first time to produce near uniformly sized and shaped Ag and Au nanoparticles at room temperature. The size of Ag and Au nanoparticles were found in the range of 25–30 nm and 1–5 nm, respectively. UV–vis spectrum, TEM and XRD measurements confirmed the formation of Ag and Au nanoparticles.
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