Structural and morphological changes in bacteria-membrane mimetic DPPE/DPPG/water systems induced by sulfadiazine |
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| Institution: | 1. Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, H-1521 Budapest, Hungary;2. Chemical Research Center – Hungarian Academy of Sciences, Institute of Surface Chemistry and Catalysis, HU-1025 Budapest, Pusztaszeri út 59-67, Hungary;3. Agrosphere Institute, Research Centre Jülich, D-52425 Jülich, Germany;1. Department of Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Joan XXIII s/n, 08028 Barcelona, Spain;2. Department of Organic Chemistry, Faculty of Chemistry, University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain;3. Laboratory of Microbiology, Faculty of Pharmacy, University of Barcelona, Joan XXIII s/n, 08028 Barcelona, Spain;1. School of Physics and Optoelectronics Engineering, Ludong University, Yantai 264025, China;2. Weifang University of Science and Technology, Shouguang 262700, China;1. Research and Development, Australian Red Cross Blood Service, Brisbane, Australia;2. Faculty of Health, Queensland University of Technology, Brisbane, Australia;1. Department of General, Visceral, and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany;2. Department of Internal Medicine I and Clinical Chemistry, University of Heidelberg, Heidelberg, Germany;1. Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia;2. Chair of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000 Ljubljana, Slovenia |
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| Abstract: | The effects of sulfadiazine (SD), one of the generally used antibiotics was studied on bacteria-membrane mimetic model systems consisting of pure dipalmitoylphosphatidylethanolamine (DPPE) and DPPE/dipalmitoylphosphatidylglycerol (DPPG) at 95/5, 80/20 and 50/50 DPPE/DPPG ratios by using differential scanning calorimetry (DSC), simultaneous small- and wide-angle X-ray scattering (SWAXS) and freeze-fracture technique. In the presence of SD, varied between 10-3 and 1 SD/lipid molar ratios, the 95/5 DPPE/DPPG system shows tendentious destruction in the layer arrangement which is accompanied by minor perturbations in the thermotropic behaviour. Moreover, at this lipid composition the addition of SD results in the formation of stacks of extremely extended flat bilayers. Systems having a higher DPPG molar ratio exhibit complex and diffuse morphologies. At 50/50 DPPE/DPPG ratio DPPG and SD act together and form large spherical vesicles. The uniform morphology is not accompanied by a regular lamellar arrangement. The range of the SD/lipid ratio, where the SD molecules are embedded into the lipid bilayers, extends to about 10-1. Over this limit the separation of SD molecules can be observed at all investigated DPPE/DPPG ratios. |
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