Poly(vinylpyrrolidone-b-styrene) block copolymers tethered surfaces for protein adsorption and cell adhesion regulation |
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| Institution: | 1. Institute of Macromolecular Chemistry AS CR, v. v. i., Heyrovsky sq. 2, Prague, Czech Republic;2. Institute of Cellular Biology and Pathology, Charles University in Prague, 1st Faculty of Medicine, Prague, Czech Republic;3. Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Prague, Czech Republic;4. Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, Oslo, Norway;1. Department of Pharmaceutics, The Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, P.O.B. 12065, Jerusalem 91120, Israel;2. Biofilm Research Laboratory, Faculty of Dental Medicine, The Hebrew University of Jerusalem, P.O.B 12272, Jerusalem 91120, Israel;3. Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.B 12, Rehovot 76100, Israel;1. Department of Materials Science and Engineering, Sharif University of Technology, Azadi Avenue, P.O. Box 11365-9466, Tehran, Iran;2. Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Azadi Avenue, P.O. Box 11365-9466, Tehran, Iran |
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| Abstract: | Poly(vinylpyrrolidone-b-styrene) (PVP-b-PS) diblock copolymers tethered to glass surfaces were prepared, and the effects on protein adsorption and cellular behavior to the glass and the modified glass surfaces investigated. The PVP-b-PS grafting process was confirmed by water contact angle and XPS measurements. The results obtained for the water contact angles suggest that there are two phases that coexist on the PVP-b-PS block copolymer tethered surface, under aqueous conditions. Although the PVP-b-PS surface possessed, to some extent, a protein resistant property, following introduction of the PS segment to the end of tethered PVP, both fibrinogen and lysozyme adsorption were increased significantly. The PVP-b-PS modified surface, based on Western-blot analysis, appeared to have the greatest amount of surface bound vitronectin, however the conformation of the adsorbed vitronectin may have subsequently been affected by the surface tethered copolymer as was suggested by cell culture results. From these results, we proposed that protein adsorption and cell adhesion can be regulated by tuning the chemical compositions of diblock copolymers tethered to surfaces. |
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