| 银杏发根的转化及其悬浮培养无性系的建立 |
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| 引用本文： | 刘树楠,孙天恩,李根保. 银杏发根的转化及其悬浮培养无性系的建立[J]. 武汉大学学报(理学版), 1998, 0(2) |
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| 作者姓名： | 刘树楠 孙天恩 李根保 |
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| 作者单位： | 武汉大学分析测试科学系 |
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| 摘 要： | 运用具有Ｒｉ质粒的发根农杆菌对银杏的叶片、幼芽和幼茎进行成功地转化，并建立银杏发根的悬浮培养无性系．冠瘿碱检测实验表明，发根农杆菌Ｒｉ质粒的Ｔ－ＤＮＡ部分已整合到银杏细胞中．这为利用生物技术开发银杏资源提供一条新的途径．
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| 关 键 词： | 银杏，发根农杆菌，发根 |
TRANSFORMATION OF GINKGO HAIRY ROOT AND ESTABLISHMENT OF ITS SUSPENSION CULTURE CLONE |
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| Liu Shunan,Sun Tianen,Li Genbao. TRANSFORMATION OF GINKGO HAIRY ROOT AND ESTABLISHMENT OF ITS SUSPENSION CULTURE CLONE[J]. JOurnal of Wuhan University:Natural Science Edition, 1998, 0(2) |
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| Authors: | Liu Shunan Sun Tianen Li Genbao |
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| Abstract: | In this thesis, Tender leaves,buds and stems of Ginkgo biloba L.were transformed successfully by Agrobacterium rhizogenes with Ri plasmid ,and its suspension culture clone was eastablished. The result of detection of opines suggested that A.rhizogenes had integrated its T DNA of the Ri plasmid into genomic DNA of ginkgo cells.A new way is offered to exploit ginkgo resources with biological technology. |
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| Keywords: | Ginkgo biloba L Agrobacterium rhizogenes hairy root |
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