| 天青A分光光度法测定硫酸软骨素含量的研究 |
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| 引用本文： | 高贵珍,焦庆才,丁一磊,陈雷. 天青A分光光度法测定硫酸软骨素含量的研究[J]. 光谱学与光谱分析, 2003, 23(3): 600-602 |
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| 作者姓名： | 高贵珍 焦庆才 丁一磊 陈雷 |
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| 作者单位： | 1. 南京大学生命科学学院,药物生物技术国家重点实验室,江苏,南京,210093;宿州师范专科学校生化系,安徽,宿州,234000
2. 南京大学生命科学学院,药物生物技术国家重点实验室,江苏,南京,210093 |
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| 摘 要： | 利用硫酸软骨素与天青A作用后 ,其复合物溶液在 6 2 5nm处吸光值与硫酸软骨素浓度成线性关系的特点 ,建立了一种测定中药复方样品中硫酸软骨素含量的快速、简便、灵敏的分光光度法。本方法在 0～ 30 μg·mL- 1 范围内呈现良好的线性关系 (R =0 999) ,回收率范围为 97 4 %～ 10 3 8%。
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| 关 键 词： | 硫酸软骨素 天青A 分光光度法 |
| 文章编号： | 1000-0593(2003)03-0600-03 |
| 修稿时间： | 2002-11-11 |
Study on Quantitative Assay of Chondroitin Sulfate with a Spectrophotometric Method of Azure A |
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| GAO Gui-zhen ,,JIAO Qing-cai ,DING Yi-lei and CHEN Lei .Pharmaceutic Biotechnology Key Laboratory,College of Life Science,Nanjing University,Nanjing ,China. Study on Quantitative Assay of Chondroitin Sulfate with a Spectrophotometric Method of Azure A[J]. Spectroscopy and Spectral Analysis, 2003, 23(3): 600-602 |
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| Authors: | GAO Gui-zhen JIAO Qing-cai DING Yi-lei CHEN Lei .Pharmaceutic Biotechnology Key Laboratory College of Life Science Nanjing University Nanjing China |
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| Affiliation: | Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China. |
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| Abstract: | A simple, accurate and rapid spectrophotmeric method was proposed for the determination of chondroitin sulfate. The method was based on the absorbances of AA-CS complex at 625 nm being in proportion to the chondroitin sulfate concentrations. The linear range was 0-30 micrograms.mL-1 (R = 0.999), and the recovery range was 97.4%-103.8%. The quantities of chondroitin sulfate in different sample were determined in this way. |
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| Keywords: | Chondroitin sulfate Azure A Spectrophotometry |
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