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基于全基因组测序的粘绿木霉Gv29-8中分泌蛋白预测
作者单位:;1.西南林业大学林学院;2.云南省森林灾害预警与控制重点实验室
摘    要:粘绿木霉Gv29-8作为木霉属中重要的生防菌之一,对该菌分泌蛋白进行预测及其特征进行明确具有重要的理论意义。利用SignalP、ProtComp等预测程序对该菌中12427条蛋白质序列进行分泌蛋白找寻,并对上述分泌蛋白的氨基酸分布、信号肽长度大小及切割位点等性质进行分析。粘绿木霉含有分泌蛋白为377个,其氨基酸长度、信号肽长度与植物病原菌不同;信号肽切割位点属于A-X-A类型,与其他已经报道的植物病原真菌、卵菌中分泌蛋白信号肽切割位点一致。

关 键 词:粘绿木霉  分泌蛋白  信号肽  预测程序

Prediction for Secreted Proteins from Trichoderma Viride Gv29-8 Genome
Institution:,College of Forestry,The Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Southwest Forestry University
Abstract:Trichoderma virens Gv29-8is the important bio-control agent of Trichoderma ssp.,which can inhibit many plant pathogens.To identify the secreted protein from T.virens and clearly it's characteristic.12,427 protein sequences in T.virens was analyzed to find the secreted protein whti the program including SignalP,ProtComp,TMHMM,big-PI Fungal predictor and TargetP.Meanwhile,the distribution of amino acids,the length of signal peptide as well as the signal peptide cleavage site of secreted protein ware analyzed.377 secrete proteins were found in T.virens.And the length of amino acids and the signal peptide were different plant pathogens.The signal peptide cleavage site belongs to AXA type,which was same as other plant pathogenic fungi,bacteria and oomycete.
Keywords:Trichoderma virens  secreted protein  signal peptide  prediction algorithm
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