Potential of short-column liquid chromatography with tandem mass spectrometric detection for the rapid study of pesticide degradation |
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Authors: | A C Hogenboom R J C A Steen W M A Niessen U A Th Brinkman |
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Institution: | (1) Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands;(2) Free University, Institute for Environmental Studies, De Boelelaan 1115, 1081 HV Amsterdam, The Netherlands;(3) HyphenMassSpec Consultancy, De Wetstraat 8, 2332 XT Leiden, The Netherlands |
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Abstract: | Summary The applicability of solid-phase extraction-LC using two short columns (SPE-LC) and/or single-short-column liquid chromatography
(SSC) combined on-line with tandem mass spectrometry (MS) was demonstrated for the rapid study of pesticide degradation. A
fast analytical procedure was developed to provide preliminary information concerning experimental conditions, approximate
rates of degradation and identity of the degradation products. Surface water samples were spiked at relevant concentration
levels with well-known microcontaminants and photolysis was used to transform parent compounds into their degradation products.
In general, the strategy was as follows: at 30-min intervals 10-mL samples were on-line enriched, separated by short-column
LC and recorded in full-scan MS to obtain information on the disappearance of the parent compound and the appearance of breakdown
products. To obtain structural information, product-ion spectra of selected compounds appearing in the full-scan MS chromatogram
were recorded; this enabled the identification of several degradation products. Total analysis time of enrichment/separation
and detection was about 10–15 min. |
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Keywords: | Column liquid chromatography Short-Column LC Environmental water samples MS-MS detection Pesticide degradation |
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