Construction of 5‐amino‐1,10‐phenanthroline‐Fe(II) Nanostructures on Glassy Carbon Electrode: Simultaneous and Selective Determination of Purine and Pyrimidine DNA Bases |
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| Authors: | Reza Karimi Shervedani Sedigheh Bagheri Dehaghi Marzieh Samiei Foroushani |
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| Institution: | Department of Chemistry, University of Isfahan, Isfahan 81746‐73441, I.R. IRAN |
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| Abstract: | 5‐amino‐1,10‐phenanthroline‐Fe(II) complex is immobilized onto GC electrode and used for determination of DNA bases. Modifications are traced by electrochemical methods. All DNA bases are electroactive on the modified electrode. The Ips increased linearly with increase of DNA bases concentration. A wide response range was observed for each base (～4 orders for guanine (GA) and adenine (A); and ～2.5 orders for thymine (T) and cytosine (C)) with DLs of 0.15, 4.44, 133.0 and 230.0 nM, respectively. The electrode was applied for determination of calf‐thymus DNA bases. The value obtained for (GA+C)/(A+T)], 0.78, is in good agreement with standard value, 0.77. |
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| Keywords: | Adenine 5‐Amino‐1 10‐phenanthroline Cytosine Guanine Thymine |
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