Electrochemical Biosensor for Naphthalene Acetic Acid in Fruits and Vegetables Based on Lipid Films with Incorporated Auxin‐binding Protein Receptor Using Graphene Electrodes |
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| Authors: | Spyridoula Bratakou Georgia‐Paraskevi Nikoleli Christina G Siontorou Stephanos Karapetis Dimitrios P Nikolelis Nikolaos Tzamtzis |
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| Institution: | 1. Laboratory of Inorganic & Analytical Chemistry, School of Chemical Engineering, Dept?1, Chemical Sciences, National Technical University of Athens, Athens, Greece;2. Laboratory of Simulation of Industrial Processes, Department of Industrial Management and Technology, School of Maritime and IndustryUniversity of Piraeus;3. ++302107274754;4. Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Athens, Greece;5. Laboratory of Inorganic Chemistry, Department of Chemistry, University of Athens, Athens, Greece |
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| Abstract: | The present work describes a miniaturized potentiometric naphthalene acetic acid (NAA) sensor on graphene nanosheets with incorporated lipid films. Auxin‐binding protein 1 receptor immobilized on the stabilized lipid films provided adequate selectivity for detection over a wide range of hormone concentrations, fast response time of ca. 5 min, and detection limit of 10 nM. The proposed sensor is easy to construct and exhibits good reproducibility, reusability, selectivity, long shelf life and high sensitivity of ca. 56 mV/decade of hormone concentration. The reliability of the biosensor was successfully evaluated using a wide range of NAA‐spiked fruits and vegetables. |
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| Keywords: | stabilized lipid films biosensor electrochemistry auxin-binding protein 1 naphthalene acetic acid |
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