Screening for Oral Cancer Using Electrochemical Telomerase Assay |
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| Authors: | Mana Hayakawa Shinobu Sato Irmina Diala Masaaki Kodama Kumiko Tomoeda‐Mori Kazuya Haraguchi Kazuhiro Tominaga Shigeori Takenaka |
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| Institution: | 1. Division of Oral and Maxillofacial Surgery, Department of Science of Physical Functions, Kyushu Dental University, Fukuoka, Japan.;2. Department of Applied Chemistry, Kyushu Institute of Technology, Fukuoka, Japan.;3. Department of Oral and Maxillofacial Surgery, National Hospital Organization Kumamoto Medical Center, Kumamoto, Japan. |
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| Abstract: | Electrochemical telomerase assay (ECTA) developed by our group was evaluated in an oral cancer screening using exfoliated oral cells and tissues obtained from patients of oral cancer, mucosa associated disease, or healthy volunteers. Telomerase activity from ECTA is correlated with hTERT mRNA expression level using a real‐time PCR and was increasing in the following order: healthy volunteer group<mucosa associated disease group<oral cancer group. Sensitivity and specificity of ECTA were 88 % and 72 %, respectively when used 17 % of the threshold value based on the receiver operating characteristic curve in ECTA data. |
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| Keywords: | telomerase hTERT gene oral cancer screening electrochemical assay |
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